DFW Breeders' Diversity Toolkit

The aim of Designing Future Wheat (DFW) Programme is to deliver new useful genetic variation and knowledge to breeding. Pre-breeding germplasm is developed and handled in a collaboration between DFW WP3 and the Germplasm Resource Unit (GRU). The new pre-breeding germplasm will include near isogenic lines (NILs) from wheat diversity sources. NILs or equivalent material are assessed for the trait of interest within DFW.
Particularly promising germplasm will be multiplied and trialed in multi-site trials at commercial breeders and scientific institutions as an annually changing Breeders' Diversity Toolkit (BTK) series. The DFW BTK series carries on from the WISP Breeders' Diversity Toolkit (see below). It is one major result of DFW, taking output from WP1 and WP2 forward to pre-breeding.

DFW Breeders' Diversity Toolkit Selection Committee

Decisions on QTL or genomic regions and germplasm are made by the Breeders Toolkit Annual Selection Committee Meeting. It decides on the content of the DFW NIL back-crossing pipeline (NIL sets, putative candidates for a BTK) and on the final selection of the annual BTK lines. The following representatives sit on the the committee:

Breeders Toolkit Select Committee

QTL or genomic regions and germplasm can be nominated by any researcher within DFW to the chair of the committee by submitting a completed form on germplasm, trait of interest, markers etc. using the following form: BTK germplasm nomination form. The committee meets in February (first time in 2018) to decide on the larger back-crossing pipeline and in September to decide on the next BTK.

The BTK will be tested in multi-site trials at different breeding companies and institutions,

DFW Breeders' Toolkit (BTK) 2019-2020

The accessions in the current selection (September 2019) are introgression of Durum, Emmer or Synthetic Wheat into Paragon or Robigus; or introgressions of wild wheat relatives into Paragon; or Near Isogenic Lines from landrace cultivars in Paragon. Each accession and controls will be planted in 3 replicates in yield trials. The following accessions are in the DFW BTK 2019-20:

Wheat Type

Accession Name

Description of Introgression

Recipient Cultivar

GRU code

WL

PW141-21-3-4-Q2A-GFP-W

Wat 1190141 QTL 2A-GFP

Paragon

WBTK0020

WL

PW292-25-6-3-Q2A-MATU-W

Wat 1190292 QTL 2A-MATU

Paragon

WBTK0021

WL

PW352-26-5-19-Q2A-DTMA-W

Wat 1190352 QTL 2A-DTMA

Paragon

WBTK0022

WL

PW468-84-4-2-Q5A-NDRE-W

Wat 1190468 QTL 5A-NDRE

Paragon

WBTK0023

WS

SHW008 SYN Sel 51

NIAB SHW-008 BC1F6

Robigus

WBTK0024

WS

SHW008 SYN Sel 61

NIAB SHW-008 BC1F6

Robigus

WBTK0025

WS

SHW090 SYN Sel 70

NIAB SHW-090 BC1F6

Robigus

WBTK0026

WR

D029 DHF1-29

Ae. mutica DH BC

Paragon

WBTK0027

WR

D329 DHF1-329

Ae. mutica DH BC

Paragon

WBTK0028

WM

gw2-A / Par (+/-)

gw2-A

Paragon

WBTK0029

WM

Par / POL (-/+)

POL

Paragon

WBTK0030

WM

gw2-A / POL (+/+)

gw2-A and POL

Paragon

WBTK0031

Control

Paragon Parent

Spring Control

W10074

Control

Robigus Parent

Winter Control

W9999

Control

Santiago Yield Standard

Winter Control

W10338

Abbreviations:
BC=back cross, DH= doubled haploid, GRU= germplasm resource centre (GRU - BTK collection), gw2-A= grain weigth 2-A allele, Par=Paragon wild type, POL=T. polonicum P1 allele (grain length), Wat= Watkins landrace, WL= derived from wheat landrace, WM= derived from wheat mutant WR= derived from wheat wild relative, WS= derived from wheat synthetic .

DFW Breeders' Toolkit 2018-2019

The accessions in the current selection (September 2018) are introgression of Durum, Emmer or Synthetic Wheat into Paragon or Robigus or Near Isogenic Lines from landrace cultivars in Paragon. Each accession and controls will be planted in 3 replicates in yield trials. The following accessions are in the DFW BTK 2018-19 (GRU codes in brackets):

Accession Description GRU code
NIAB-TK-1746 Karim Durum Wheat introgression into Paragon WS0087
NIAB-TK-1549: Synthetic Hexaploid Wheat-18 introgression into Robigus WS0063
NIAB-TK-1604: Tios Emmer Landrace introgression into Robigus WS0076
PW34.12 Q1A-HTlandrace allele from Watkins 1190034 WL0105
PW34.12 Q1A-HTcomparative Paragon allele WL0104
PW468.84 Q5A GRYLDlandrace allele from Watkins 1190468 WL0292
PW468.84 Q5A GRYLDcomparative Paragon allele WL0291
Paragonparental control
Robigusparental control
Santiagoindustry standard

DFW Breeders' Toolkit (BTK) 2017-2018

The genotypes of the DFW-BTK were Near Isogenic Lines either derived from landrace cultivars (two NIL streams from former WISP) or from an EMS mutant (one NIL stream from WP1).

To obtain seed contact Simon Orford, Germplasm Resources Unit, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH at simon.orford@jic.ac.uk

Normally, 500g of each line requested will be dispatched upon receipt of signed MTA. In the event of seed stock exhaustion, seed will be provided after regeneration at GRU.


Axiom Genotypes of Toolkit NILs

Many of the toolkit NILs, candidates for the BTK, have been genotyped using the 35k bread wheat genotyping array. Genotypes can be inspected at:

WISP Breeders' Toolkit (BTK) 2016-2017

The WISP Breeders' Diversity Toolkit, based on landrace cultivar diversity, is the result of joint resource development and analysis carried out at the John Innes Centre, Rothamsted Research, University of Bristol, and University of Nottingham. The genotypes in the current selection (as of January 2015) are derived from F4 SSD, progeny of crosses between the UK spring wheat variety 'Paragon' and AE Watkins landrace cultivars[1] kept at the John Innes Centre.

The main prebreeding output of the WISP landrace pillar is Near Isogenic Lines in the genetic background of Paragon. They were developed through two marker assisted backcrosses which started off with a RIL from the respective Paragon x Watkins landrace population. So the NILs carrying Watkins alleles will carry ~ 87% Paragon background. This is the first cohort of landrace derived NILs from WISP. This is why the emphasis is on height and heading as the QTL analysis to inform the choices were based on data from the first season of WISP at JIC. However, the first year of assessment of the NILs (14-15) gave us some encouragement that landrace alleles might carry agronomic benefit and we encourage you to assess these materials.

WISP Breeders' Diversity Toolkit 2016

Loci covered are on chromosomes 3A and 5A for ear emergence, and 7B for height.

The set is a subset of the 2015 Toolkit, lines for which the last field trial suggested that the QTL loci confer a yield benefit upon Paragon.

WISP Breeders' Diversity Toolkit 2015

Loci covered are on chromosomes 1B, 2D, 3A, 5A (x2) and 6A for ear emergence, 5A, 6A (x2) and 7B for height and 2D for Grain Yield.

The 2015 WISP Breeders' Diversity Toolkit information can be downloaded here:

WISP Breeders' Diversity Toolkit 2014

Information on the 2014 toolkit is available here.

 


References

[1] Wingen LU, Orford S, Goram R, Leverington-Waite M, Bilham L, et al. (2014) Establishing the AE Watkins landrace cultivar collection as a resource for systematic gene discovery in bread wheat. Theoretical and Applied Genetics 127: 1831-1842.