DFW Breeders' Diversity Toolkit

The DFW (Designing Future Wheat) Breeders' Diversity Toolkit carries on from the WISP Breeders' Diversity Toolkit (below), but will also include NILs from other diversity sources than just landraces. It is one major result coordinated by Work Package 3 (WP3) of the DFW programme, taking output from WP1 and WP2 forward to pre-breeding.

DFW Breeders' Diversity Toolkit 2017

The genotypes in the current selection (September 2017) are Near Isogenic Lines either derived from landrace cultivars (two NIL streams from former WISP) or from an EMS mutant (one NIL stream from WP1).

To obtain seed contact Simon Orford, Germplasm Resources Unit, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH at simon.orford@jic.ac.uk

Normally, 500g of each line requested will be dispatched upon receipt of signed MTA. In the event of seed stock exhaustion, seed will be provided after regeneration at GRU.

WISP Breeders' Diversity Toolkit

The WISP Breeders' Diversity Toolkit, based on landrace cultivar diversity, is the result of joint resource development and analysis carried out at the John Innes Centre, Rothamsted Research, University of Bristol, and University of Nottingham. The genotypes in the current selection (as of January 2015) are derived from F4 SSD, progeny of crosses between the UK spring wheat variety 'Paragon' and AE Watkins landrace cultivars[1] kept at the John Innes Centre.

The main prebreeding output of the WISP landrace pillar is Near Isogenic Lines in the genetic background of Paragon. They were developed through two marker assisted backcrosses which started off with a RIL from the respective Paragon x Watkins landrace population. So the NILs carrying Watkins alleles will carry ~ 87% Paragon background. This is the first cohort of landrace derived NILs from WISP. This is why the emphasis is on height and heading as the QTL analysis to inform the choices were based on data from the first season of WISP at JIC. However, the first year of assessment of the NILs (14-15) gave us some encouragement that landrace alleles might carry agronomic benefit and we encourage you to assess these materials.

WISP Breeders' Diversity Toolkit 2016

Loci covered are on chromosomes 3A and 5A for ear emergence, and 7B for height.

The set is a subset of the 2015 Toolkit, lines for which the last field trial suggested that the QTL loci confer a yield benefit upon Paragon.

WISP Breeders' Diversity Toolkit 2015

Loci covered are on chromosomes 1B, 2D, 3A, 5A (x2) and 6A for ear emergence, 5A, 6A (x2) and 7B for height and 2D for Grain Yield.

The 2015 WISP Breeders' Diversity Toolkit information can be downloaded here:

WISP Breeders' Diversity Toolkit 2014

Information on the 2014 toolkit is available here.



[1] Wingen LU, Orford S, Goram R, Leverington-Waite M, Bilham L, et al. (2014) Establishing the AE Watkins landrace cultivar collection as a resource for systematic gene discovery in bread wheat. Theoretical and Applied Genetics 127: 1831-1842.