Breeders' Toolkit | Landrace Pillar of the Wheat Pre-breeding LOLA /Wheat Improvement Strategic Programme/ consortium

DFW Breeders' Diversity Toolkit

The aim of Designing Future Wheat (DFW) Programme is to deliver new useful genetic variation and knowledge to breeding. Pre-breeding germplasm is developed and handled in a collaboration between DFW WP3 and the Germplasm Resource Unit (GRU). The new pre-breeding germplasm will include near isogenic lines (NILs) from wheat diversity sources. NILs or equivalent material are assessed for the trait of interest within DFW.
Particularly promising germplasm will be multiplied and trialed in multi-site trials at commercial breeders and scientific institutions as an annually changing Breeders' Diversity Toolkit (BTK) series. The DFW BTK series carries on from the WISP Breeders' Diversity Toolkit (see below). It is one major result of DFW, taking output from WP1 and WP2 forward to pre-breeding.

DFW Breeders' Diversity Toolkit Selection Committee

Decisions on QTL or genomic regions and germplasm are made by the Breeders Toolkit Annual Selection Committee Meeting. It decides on the content of the DFW NIL back-crossing pipeline (NIL sets, putative candidates for a BTK) and on the final selection of the annual BTK lines. The following representatives sit on the the committee:

QTL or genomic regions and germplasm can be nominated by any researcher within DFW to the chair of the committee by submitting a completed form on germplasm, trait of interest, markers etc. using the following form: BTK germplasm nomination form. The committee meets in February (first time in 2018) to decide on the larger back-crossing pipeline and in September to decide on the next BTK.

The BTK will be tested in multi-site trials at different breeding companies and institutions,

Summary of all accessions grown in BTK trials from 2017-2022

Download a spreadsheet with the accession names from all Breeders’ Toolkit trials up to 2022:

Download 2021 spreadsheet

DFW Breeders’ Toolkit (BTK) 2021-2022

The accessions in the September 2021 selection are: a) introgressions of Synthetic Wheat into Robigus (WS); b) BC3 Near Isogenic Lines from mutant wheat into Paragon (WM); c) introgressions of Ae. Mutica (wild wheat relative) into Paragon (WR); e) Near Isogenic Lines from landrace cultivars in Paragon (WL). Not all lines are grown at all seven Breeders' sites. Accessions and controls were planted in mostly three replicates in yield trials.

Type (Wheat)	Accession Name	Description	Recipent Cultivar	BTK Code	Breed. sites
WS	SHW095-SEL 0454	donor NIAB SHW095	Robigus	WBTK0049	7
WS	SHW035-SEL 0302	donor NIAB SHW035	Robigus	WBTK0050	7
WS	SHW089-SEL 0385	donor NIAB SHW089	Robigus	WBTK0051	7
WS	SHW027-SEL 0279	donor NIAB SHW027	Robigus	WBTK0052	7
WS	SHW080-SEL 0435	donor NIAB SHW080	Robigus	WBTK0053	7
WS	SHW082-SEL 0338	donor NIAB SHW082	Robigus	WBTK0054	7
WM	GPC-B1_WT GW2-ABD_WT	WT (BC3)	Paragon	WBTK0055	4
WM	gpc-B1_Mut GW2-ABD_WT	GPC-B1 funct	Paragon	WBTK0056	7
WM	GPC-B1_WT gw2-ABD_Mut	gw2-triple + GPC-B1 non-funct	Paragon	WBTK0057	4
WM	gpc-B1_Mut gw2-ABD_Mut	gw2-triple + GPC-B1 funct	Paragon	WBTK0058	7
WR	NU DHF1-006 T.ae. x Ae. mutica	BC DH	Paragon	WBTK0059	7
WR	NU DHF1-011 T.ae. x Ae. mutica	BC DH	Paragon	WBTK0060	4
WR	NU DHF1-010 T.ae. x Ae. mutica	BC DH	Paragon	WBTK0061	4
WR	NU DHF1-008 T.ae. x Ae. mutica	BC DH	Paragon	WBTK0062	4
WR	NU DHF1-013 T.ae. x Ae. mutica	BC DH	Paragon	WBTK0063	4
WL	ParW292-54-8-2-39-Q5B-PH-W	Wat292 QTL 5B-PH	Paragon	WBTK0064	4
WL	ParW292-54-8-2-43-Q5B-PH-P	Wat292 QTL 5B-PH	Paragon	WBTK0065	4
WL	ParW475-31-4-6-3-Q5B-GW-W	Wat475 QTL 5B-GW	Paragon	WBTK0066	4
WL	ParW475-31-4-6-15-Q5B-GW-P	Wat475 QTL 5B-GW	Paragon	WBTK0067	4
WL	ParW387-15-13-2-6-Q4A-GW-W	Wat387 QTL 4A-GW	Paragon	WBTK0068	4
WL	ParW387-15-13-2-16-Q4A-GW-P	Wat387 QTL 4A-GW	Paragon	WBTK0069	4
WL	ParW496-41-12-4-14-Q2D-PH-W	Wat496 QTL 2D-PH	Paragon	WBTK0070	4
WL	ParW496-41-12-4-7-Q2D-PH-P	Wat496 QTL 2D-PH	Paragon	WBTK0071	4
WL	ParW141-4-5-1-15-Q6B-GY-W	Wat141 QTL 6B-GY	Paragon	WBTK0072	4
WL	ParW141-4-5-1-2-Q6B-GY-P	Wat141 QTL 6B-GY	Paragon	WBTK0073	4
WL	ParW199-6-9-6-21-Q6B-NDVI-W	Wat199 QTL 6B-NDVI	Paragon	WBTK0074	4
WL	ParW199-6-9-6-38-Q6B-NDVI-P	Wat199 QTL 6B-NDVI	Paragon	WBTK0075	4
WL	ParW032-78-7-14-10-Q4D-GY-W	Wat032 QTL 4D-GY	Paragon	WBTK0076	4
WL	ParW032-78-7-14-11-Q4D-GY-P	Wat032 QTL 4D-GY	Paragon	WBTK0077	4
WL	ParW468-34-12-7-19-Q6A-HI-W	Wat468 QTL 6A-HI	Paragon	WBTK0078	1
WL	ParW468-34-12-7-5-Q6A-HI-P	Wat468 QTL 6A-HI	Paragon	WBTK0079	1
WL	ParW199-22-5-4-10-Q2B-HI-W	Wat199 QTL 2B-HI	Paragon	WBTK0080	1
WL	ParW199-22-5-4-15-Q2B-HI-P	Wat199 QTL 2B-HI	Paragon	WBTK0081	1
WL	ParW034-13-2-7-17-Q2A-BM-W	Wat034 QTL 2A-BM	Paragon	WBTK0082	1
WL	ParW034-13-2-7-22-Q2A-BM-P	Wat034 QTL 2A-BM	Paragon	WBTK0083	1
WL	ParW398-88-13-5-9-Q6B-Hd-W	Wat398 QTL 6B-Hd	Paragon	WBTK0084	1
WL	ParW398-88-13-5-15-Q6B-Hd-P	Wat398 QTL 6B-Hd	Paragon	WBTK0085	1
WL	ParW209-42-4-2-16-Q6A-BM-W	Wat209 QTL 6A-BM	Paragon	WBTK0086	1
WL	ParW209-42-4-2-13-Q6A-BM-P	Wat209 QTL 6A-BM	Paragon	WBTK0087	1
WL	ParW292-91-1-3-18-Q3D-BM-W	Wat292 QTL 3D-BM	Paragon	WBTK0088	1
WL	ParW292-91-1-3-11-Q3D-BM-P	Wat292 QTL 3D-BM	Paragon	WBTK0089	1
WL	ParW246-83-4-5-7-Q1B-NDVI-W	Wat246 QTL 1B-NDVI	Paragon	WBTK0090	1
WL	ParW246-83-4-5-15-Q1B-NDVI-P	Wat246 QTL 1B-NDVI	Paragon	WBTK0091	1
WL	ParW199-59-6-8-12-Q7A-BM-W	Wat199 QTL 7A-BM	Paragon	WBTK0092	1
WL	ParW199-59-6-8-10-Q7A-BM-P	Wat199 QTL 7A-BM	Paragon	WBTK0093	1
Control	Paragon	Spring Control		WBTK-PAR1	7
Control	Robigus	Winter Control		WBTK-ROB1	7
Control	Santiago	Winter Control		WBTK-SAN1	7

DFW Breeders’ Toolkit (BTK) 2020-2021

The accessions in the September 2020 selection are: a) introgressions of Synthetic Wheat into Robigus (WS); b) introgression of Ae. Mutica (wild wheat relative) into Paragon (WR); c) Near Isogenic Lines from landrace cultivars in Paragon (WL); or e) Near Isogenic Lines from mutant wheat into Paragon (WM). Accessions and controls were planted in 3 replicates in yield trials.

Type (Wheat)	Accession Name	Description	Recipent Cultivar	GRU Code
WS	SHW-075Bc1 F6_SEL165	SHW-075Bc1 F6_SEL165	Robigus	WBTK0041
WS	SHW-008Bc1 F7_SEL63	SHW-008Bc1 F7_SEL63	Robigus	WBTK0040
WS	SHW-008Bc1 F7_SEL57	SHW-008Bc1 F7_SEL57	Robigus	WBTK0039
WS	SHW-008Bc1 F7_SEL50B	SHW-008Bc1 F7_SEL50B	Robigus	WBTK0038
WS	DFW SEL 0011B	DFW SEL 0011B	Robigus	WBTK0036
WS	DFW SEL 0015C	DFW SEL 0015C	Robigus	WBTK0036
WR	DHF1-196 T. aestivum x Ae. Mutica	Ae. mutica DH BC	Paragon	WBTK0046
WL	PW292-93-7-13-Q1B-MATU-W	Wat292 QTL 1B-MAT	Paragon	WBTK0045
WL	PW292-93-7-16-Q1B-MATU-P	Wat292 QTL 1B-MAT	Paragon	WBTK0044
WL	PW352-26-2-19-Q5D-MATU-P	Wat352 QTL 5D-MAT	Paragon	WBTK0042
WL	PW352-26-2-2-Q5D-MATU-W	Wat352 QTL 5D-MAT	Paragon	WBTK0043
WM	Paragon (BC4) (gw2-ABD_Mut)	TaGW2 triple knock-out	Paragon	WBTK0047
WM	Paragon (BC4) (GW2_ABD_WT)	TaGW2 wild-type	Paragon	WBTK0048
Control	Paragon	Spring Control		W10074
Control	Santiago	Winter Control, Yield Standard		W10338
Control	Robigus	Winter Control		W9999

BC=back cross, DH= doubled haploid, GRU= germplasm resource centre (GRU– BTK collection), Par=Paragon wild type, Wat= Watkins landrace, WL= derived from wheat landrace, WM= derived from wheat mutant WR= derived from wheat wild relative, WS= derived from wheat synthetic.

DFW Breeders' Toolkit (BTK) 2019-2020

The accessions in the current selection (September 2019) are: introgression of Durum, Emmer or Synthetic Wheat into Paragon or Robigus; or introgressions of wild wheat relatives into Paragon; or Near Isogenic Lines from landrace cultivars in Paragon. Each accession and controls will be planted in 3 replicates in yield trials. The following accessions are in the DFW BTK 2019-20:

Wheat Type	Accession Name	Description of Introgression	Recipient Cultivar	GRU code
WL	PW141-21-3-4-Q2A-GFP-W	Wat141 QTL 2A-GFP	Paragon	WBTK0020
WL	PW292-25-6-3-Q2A-MATU-W	Wat292 QTL 2A-MATU	Paragon	WBTK0021
WL	PW352-26-5-19-Q2A-DTMA-W	Wat352 QTL 2A-DTMA	Paragon	WBTK0022
WL	PW468-84-4-2-Q5A-NDRE-W	Wat468 QTL 5A-NDRE	Paragon	WBTK0023
WS	SHW008 SYN Sel 51	NIAB SHW-008 BC1F6	Robigus	WBTK0024
WS	SHW008 SYN Sel 61	NIAB SHW-008 BC1F6	Robigus	WBTK0025
WS	SHW090 SYN Sel 70	NIAB SHW-090 BC1F6	Robigus	WBTK0026
WR	D029 DHF1-29	Ae. mutica DH BC	Paragon	WBTK0027
WR	D329 DHF1-329	Ae. mutica DH BC	Paragon	WBTK0028
WM	gw2-A / Par (+/-)	gw2-A	Paragon	WBTK0029
WM	Par / POL (-/+)	POL	Paragon	WBTK0030
WM	gw2-A / POL (+/+)	gw2-A and POL	Paragon	WBTK0031
Control	Paragon Parent	Spring Control	W10074
Control	Robigus Parent	Winter Control	W9999
Control	Santiago Yield Standard	Winter Control	W10338

Abbreviations:
BC=back cross, DH= doubled haploid, GRU= germplasm resource centre (GRU - BTK collection), gw2-A= grain weigth 2-A allele, Par=Paragon wild type, POL=T. polonicum P1 allele (grain length), Wat= Watkins landrace, WL= derived from wheat landrace, WM= derived from wheat mutant WR= derived from wheat wild relative, WS= derived from wheat synthetic .

DFW Breeders' Toolkit 2018-2019

The accessions in the current selection (September 2018) are introgression of Durum, Emmer or Synthetic Wheat into Paragon or Robigus or Near Isogenic Lines from landrace cultivars in Paragon. Each accession and controls will be planted in 3 replicates in yield trials. The following accessions are in the DFW BTK 2018-19 (GRU codes in brackets):

Accession	Description	GRU code
NIAB-TK-1746	Karim Durum Wheat introgression into Paragon	WS0087
NIAB-TK-1549:	Synthetic Hexaploid Wheat-18 introgression into Robigus	WS0063
NIAB-TK-1604:	Tios Emmer Landrace introgression into Robigus	WS0076
PW34.12 Q1A-HT	landrace allele from Watkins 1190034	WL0105
PW34.12 Q1A-HT	comparative Paragon allele	WL0104
PW468.84 Q5A GRYLD	landrace allele from Watkins 1190468	WL0292
PW468.84 Q5A GRYLD	comparative Paragon allele	WL0291
Paragon	parental control
Robigus	parental control
Santiago	industry standard

DFW Breeders' Toolkit (BTK) 2017-2018

The genotypes of the DFW-BTK were Near Isogenic Lines either derived from landrace cultivars (two NIL streams from former WISP) or from an EMS mutant (one NIL stream from WP1).

Information and statistics on the result of WISP BTK 2016 and the new varieties in the DFW 2017-2018 Toolkit: DFW_WP3_Breeders_Toolkit_2017.pdf

To obtain seed browse SeedStor:
Breeders Toolkit Collection at SeedStor (GRU)
or the larger Academic Toolkit collection:
Academic Wheat Toolkit Collection at SeedStor (GRU)

For questions contact Simon Orford, Germplasm Resources Unit, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH at simon.orford@jic.ac.uk

Axiom Genotypes of the Academic Toolkit Landrace NILs

Many of the landrace NILs have been genotyped using the 35k bread wheat genotyping array. Genotypes can be inspected at:

WISP Breeders' Toolkit (BTK) 2016-2017

The WISP Breeders' Diversity Toolkit, based on landrace cultivar diversity, is the result of joint resource development and analysis carried out at the John Innes Centre, Rothamsted Research, University of Bristol, and University of Nottingham. The genotypes in the current selection (as of January 2015) are derived from F4 SSD, progeny of crosses between the UK spring wheat variety 'Paragon' and AE Watkins landrace cultivars[1] kept at the John Innes Centre.

The main prebreeding output of the WISP landrace pillar is Near Isogenic Lines in the genetic background of Paragon. They were developed through two marker assisted backcrosses which started off with a RIL from the respective Paragon x Watkins landrace population. So the NILs carrying Watkins alleles will carry ~ 87% Paragon background. This is the first cohort of landrace derived NILs from WISP. This is why the emphasis is on height and heading as the QTL analysis to inform the choices were based on data from the first season of WISP at JIC. However, the first year of assessment of the NILs (14-15) gave us some encouragement that landrace alleles might carry agronomic benefit and we encourage you to assess these materials.

WISP Breeders' Diversity Toolkit 2016

Loci covered are on chromosomes 3A and 5A for ear emergence, and 7B for height.

The set is a subset of the 2015 Toolkit, lines for which the last field trial suggested that the QTL loci confer a yield benefit upon Paragon.

Information and statistics on the 2016 Toolkit: WISP_landrace_pillar_Breeders_Toolkit_2016.pdf
Information and statistics on the result of WISP 2016 BTK and the new varieties in the DFW 2017 Toolkit: DFW_WP3_Breeders_Toolkit_2017.pdf

WISP Breeders' Diversity Toolkit 2015

Loci covered are on chromosomes 1B, 2D, 3A, 5A (x2) and 6A for ear emergence, 5A, 6A (x2) and 7B for height and 2D for Grain Yield.

The 2015 WISP Breeders' Diversity Toolkit information can be downloaded here:

Information on the 2015 Toolkit: WISP_landrace_pillar_Breeders_Toolkit_2015.pdf
Statistics on the 2015 Toolkit: WISP_landrace_pillar_Breeders_Toolkit_2015_NILs_statistics.xls

WISP Breeders' Diversity Toolkit 2014

Information on the 2014 toolkit is available here.

References

[1] Wingen LU, Orford S, Goram R, Leverington-Waite M, Bilham L, et al. (2014) Establishing the AE Watkins landrace cultivar collection as a resource for systematic gene discovery in bread wheat. Theoretical and Applied Genetics 127: 1831-1842.

DFW WP3 Landraces